What is the use of rhodamine B?
What is the use of rhodamine B?
As one of the most commonly used dyes, Rhodamine B (RhB) is widely used in industrial purposes, such as printing and dyeing in textile, paper, paints, leathers etc. However, the organic dyes will cause serious environmental and biological problems, even capable to induce irritation to the skin, eyes.
What type of dye is rhodamine B?
fluorescent xanthene dye
Another pollutant that is extensively used for dyeing purpose is Rhodamine B (RhB). It is a water-soluble fluorescent xanthene dye used to dye various substances.
Is rhodamine B an azo dye?
Rhodamine dye. dyes, half the number belong to the class of Azo dyes. Technology, P.O. Box 652, Cape Town 8000, South Africa.
What is the composition of rhodamine B?
Rhodamine B is an organic chloride salt having N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium as the counterion. An amphoteric dye commonly used as a fluorochrome. It has a role as a fluorochrome, a fluorescent probe and a histological dye.
How do you test for Rhodamine B?
Accurate analysis techniques are necessary in order to avoid potential health risks. Merck’s Purospher® HPLC columns are ideal for the detection of Rhodamine B in chili extract. The columns offer highly reliable results, with excellent separations and outstanding peak symmetry.
Is Rhodamine B an acid or base?
Abstract. Fluorescence lifetime measurements of rhodamine B in ethanol solution have confirmed the presence of two distinct species dependent on acidity. Rotational diffusion times of these species are identical and we propose that they are the acid and base forms of the dye.
Is Rhodamine B water soluble?
Rhodamine B base is also used as a laser dye, tunable around 610 nm with the fluorescence yield being temperature dependent. Solubility : Soluble in ethanol (1 mg/ml), methanol, chloroform, and DMF. Insoluble in water.
Why is rhodamine B toxic?
The rhodamine series of xanthene dyes are highly fluorescent and should not be, therefore, very toxic due to light catalyzed re- actions. The toxicity observed in populations of insects that had been fed on either rhodamine B or rhodamine 6G and subsequently been held in the dark (YOHO et al.
How do you make Rhodamine B?
Stock Standard 1 Dissolve 0.01 g of rhodamine B standard in 10 mL of DI water. The concentration of Stock Standard 1 will be 1000 μg/mL. Stock Standard 2 Dilute 200 μL of Stock Standard 1 to 10 mL with DI water. The concentration of Stock Standard 2 will be 20 μg/mL.
What is Rhodamine B base?
Application: Rhodamine B base is a fluorescent biological staining dye. CAS Number: 509-34-2. Molecular Weight: 442.56. Molecular Formula: C28H30N2O3.
Is Rhodamine B cationic or anionic?
In addition, other cationic dyes, like malachite green (MG) and rhodamine B (RhB), as well as anionic dyes, like indigo carmine (IC), acid fuchsine (AF), acid orange (AO), and fluorescein disodium salt (FD), are also tested in this work.
What is Rhodamine B isothiocyanate used for?
Rhodamine B isothiocyanate (RBITC) is a fluorescent dye. It acts as an anterograde and retrograde marker of retinal neurons in the adult rat. Rhodamine B isothiocyanate has been used as a fluorescent probe to label pathogenic bacterium Staphylococcus aureus and is also used to label type I collagen (C1).
Is Rhodamine B adsorbed from ethanolic solution?
(c) Rhodamine B physically adsorbed from ethanolic solutions and subsequent removal of solvent by evaporation. These results show that the positive charge density on the nitrogen atom is smaller for dyed samples when compared with the adsorbed ones.
What is the application of rhodamine dye?
Rhodamine dyes are also used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. It is also used in rose milk, a popular Indian beverage.
What is the fluorescence yield of rhodamine B?
Rhodamine B is tunable around 610 nm when used as a laser dye. Its luminescence quantum yield is 0.65 in basic ethanol, 0.49 in ethanol, 1.0, and 0.68 in 94% ethanol. The fluorescence yield is temperature dependent; the compound is fluxional in that its excitability is in thermal equilibrium at room temperature.