# What is the difference between RT and RRT in HPLC? ## What is the difference between RT and RRT in HPLC?

In high pressure liquid chromatography (HPLC), the compound is injected through a column of different sized beads. The amount of time it takes for the compound to pass through the column is the retention time (RT). The relative retention time (RRT) is the comparison of the RT of one compound to another.

## How is retention factor calculated?

In thin-layer chromatography, the retention factor (Rf) is used to compare and help identify compounds. The Rf value of a compound is equal to the distance traveled by the compound divided by the distance traveled by the solvent front (both measured from the origin).

How do you calculate corrected refractive index?

Index of refraction formula Index of refraction of any medium is defined as the proportion between the speed of light in vacuum and in the investigated medium. The refractive index equation is: n = c / v.

What is an Rf value?

### What is the Kovats index?

The Kovats index applies to organic compounds. The method interpolates peaks between bracketing n -alkanes. Kovats index of n-alkanes is 100 times their carbon number, e.g. the Kovats index of n -Butane is 400. The Kovats index is dimensionless, contrary to retention time or retention volume.

### How do you calculate the Kovats index of alkanes?

Kovats index (I) is calculated as I= 100 [n + (N – n) x (Log tr (unknown) – logtr (n))/ logtr (N) – logtr (n)) where n equals the number of carbon atoms in the smaller alkane, N is the number of carbon atoms of the larger alkane, tr is in all cases the adjusted retention time (calculated at time – time of the undelayed small compound).

What is Kovats retention index in gas chromatography?

In gas chromatography, Kovats retention index (shorter Kovats index, retention index; plural retention indices) is used to convert retention times into system-independent constants.

How to identify peaks in a table of retention indices?

Tables of retention indices are used to identify peaks by comparing measured retention indices with the tabulated values. The Kovats index applies to organic compounds. The method interpolates peaks between bracketing n -alkanes. Kovats index of n-alkanes is 100 times their carbon number, e.g. the Kovats index of n -Butane is 400.

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