What is lactophenol cotton blue used for?

Published by Anaya Cole on

What is lactophenol cotton blue used for?

Lactophenol cotton blue wet mount preparation is commonly used for microscopic identification of fungi. It is formulated with lactophenol, which serves as the mounting fluid and the dye, cotton blue. Lactic acid preserves the fungal structure and clears the tissue while phenol acts as a disinfectant.

What is lactophenol cotton blue staining?

Lactophenol cotton blue (LCB) is a mixture of methyl blue, a histological stain, and lactophenol (a solution of phenol, lactic acid, and glycerol in water). It is used in wet-mount preparations for visualization of fungal structures, especially in medical mycology.

What are the compositions of lactophenol cotton blue solution?

The preparation has three components: phenol, which will kill any live organisms; lactic acid which preserves fungal structures, and cotton blue which stains the chitin in the fungal cell walls.

How do you make cotton blue Lactophenol?

A preparation of 50ml Lactophenol cotton Blue staining solution is made up of:

  1. Distilled water 50ml.
  2. Cotton Blue (Aniline Blue) 0.125g.
  3. Phenol Crystals (C6H5O4) 50g.
  4. Glycerol 100ml.
  5. Lactic acid (CH3CHOH COOH) 50ml.
  6. 70% ethanol.

How do you make iodine-glycerol solution?

A iodine-glycerol solution prepared by a take 1.0 gm Lugols Iodine and 2.0 gm Potassium Iodine and dissolve into 100 ml distilled water, mix well and keep in a brown reagent bottle [10] Evaluation of Iodine-Glycerol for Wet-Mount Preparation of Feces for Detection of Intestinal Parasites.

Why is lactophenol used for mold?

Lactophenol cotton blue is a stain that is used to examine fungal elements following either a tape preparation or a scraping. This stain contains phenol, which will kill the organisms, lactic acid which preserves fungal structures, and cotton blue which stains the chitin found in the fungal cell walls.

What is the use of lactophenol in simple staining?

The lactophenol cotton blue solution acts as a mounting solution as well as a staining agent. The solution is clear and blue in color and it is made up of a combination of three main reagents: Phenol: It acts as a disinfectant by killing any living organisms. Lactic acid: To preserve the fungal structures.

What are the four major components of Lactophenol cotton blue?

Lactophenol Cotton Blue (LPCB).

  • Cotton blue (Aniline blue) 0.05 g.
  • Phenol crystals (C6H5O4) 20 g.
  • Glycerol 40 mL.
  • Lactic acid (CH3CHOH COOH) 20 mL.
  • Distilled water 20 mL.

Does cotton blue stain bacteria?

Dark blue rings and circles emerged when the non-specific polysaccharide stain lactophenol cotton blue was added to Gram stained slides. The dark blue staining is attributable to the presence of capsular polysaccharides and bacterial slime associated with clumps of Gram-negative bacteria.

How do you make a blue cotton solution?

What is the function in glycerol in the composition of Lactophenol cotton blue stain?

method used in the laboratory. Main components of lactophenol cotton blue are phenol, lactic acid,glycerol and cotton blue. Phenol acts as a disinfectant, lactic acid preserves the morphology of fungus, glycerol acts as a hygroscopic agent which prevents drying and cotton blue stains the outer wall of fungus.

How does Lactophenol aniline blue work?

Remel Lactophenol Aniline Blue is a stain recommended for use in wet mount preparations for microscopic examination of fungi. Phenol precipitates cytoplasmic proteins and inactivates enzyme systems within the fungal cell. Glycerol prevents drying, allowing for examination of the stain 18-24 hours after preparation.

How does lactophenol aniline blue work?

What is the function in glycerol in the composition of lactophenol cotton blue stain?

What is function of iodine glycerol in fungi morphology?

In iodine-glycerol method, iodine acts as fungicide as well as an agent for staining fungal structures. Glycerol prevents rapid drying of the wet preparation.

Can Lactophenol cotton blue stain bacteria?

Abstract. Dark blue rings and circles emerged when the non-specific polysaccharide stain lactophenol cotton blue was added to Gram stained slides. The dark blue staining is attributable to the presence of capsular polysaccharides and bacterial slime associated with clumps of Gram-negative bacteria.

How do you make cotton blue lactophenol?

Why is Lactophenol used for mold?

What does lactophenol cotton blue stain look like?

The lactophenol cotton blue solution acts as a mounting solution as well as a staining agent. The stain will give the fungi a blue-colored appearance of the fungal spores and structures, such as hyphae.

How do you use lactophenol cotton blue to stain fungi?

The lactophenol cotton blue solution acts as a mounting solution as well as a staining agent. The stain will give the fungi a blue-colored appearance of the fungal spores and structures, such as hyphae. A preparation of 50ml Lactophenol cotton Blue staining solution is made up of:

What is lactophenol cotton blue solution used for?

The lactophenol cotton blue solution acts as a mounting solution as well as a staining agent. The solution is clear and blue in color and it is made up of a combination of three main reagents: Phenol: It acts as a disinfectant by killing any living organisms Lactic acid: To preserve the fungal structures

How long does it take for lactophenol cotton blue to dissolve?

Lactophenol Cotton Blue solution is prepared for over two days leaving the reagents undisturbed to allow dissolving and maturation. Day 1: Dissolve the cotton blue in distilled water and leave to rest overnight. This eliminates insoluble dye.

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