Does BamHI cut DNA?
Does BamHI cut DNA?
In addition to the canonical G decreases from G-A-T-C-C site, BamHI cuts DNA at several sites that we have named noncanonical BamHI. 1 sites. The number of BamHI. 1 sites in simian virus 40 and pBR322 was determined to be 13 for each DNA.
What is the source of BamHI restriction enzyme?
BamHI is a type II restriction enzyme derived from Bacillus amyloliquefaciens. Like all Type II restriction endonucleases, it is a dimer and the recognition site is palindromic and 6 bases in length. It recognizes the DNA sequence of G’GATCC and leaves an overhang of GATC which is compatible with many other enzymes.
How do you inactivate bamh1?
Only small amounts of BamHI (up to 10 units) can be inactivated at 80°C in 20 min. To prepare the digested DNA for electrophoresis: – stop the digestion reaction by adding 0.5 M EDTA, pH 8.0 (#R1021), to achieve a 20 mM final concentration. Mix thoroughly, add an electrophoresis loading dye and load onto gel.
Where can I find more details about BSA-free versions of BamHI?
Find more details at www.neb.com/BSA-free. BamHI has a High Fidelity version BamHI-HF ® ( NEB #R3136 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.
What is a unit of BamHI?
A E. coli strain that carries the BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). One unit is defined as the amount of BamHI required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl..
What is BglII enzyme?
An E. coli strain that carries the BglII gene from Bacillus globigii (ATCC 49760). One unit is defined as the amount of BglII required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Not sensitive to CpG, dcm, or dam methylation. Why Choose Recombinant Enzymes? How can this enzyme be inactivated?
What is 1 unit of BglII?
One unit is defined as the amount of BglII required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Not sensitive to CpG, dcm, or dam methylation.